Radioiodination of sulfhydryl-sensitive proteins
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چکیده
منابع مشابه
Radioiodination of sulfhydryl-sensitive proteins.
Radioiodination with rz51 has emerged as the method of choice for labeling protein to high specific radioactivity. A number of procedures have been developed to iodinate proteins under mild conditions and with reproducible results (l-7). Of these techniques, none is suitable for the iodination of proteins having thiol groups. Exposure to the oxidants Nchloro-p-toluene sulfonamide (chloramine-T)...
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Egg albumin in the native, unaltered state, does not give tests characteristic of sulfhydryl groups. When, however, this protein is treated in any one of several ways, such as by heat (8, 9, 17), ultraviolet irradiation (lo), shaking (lo), or by solution in urea or other amides (7), free sulfhydryl groups make their appearance. The amount of free -SH groups appearing in egg albumin through the ...
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Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mild detergent treatment and subsequent recovery of immune complexes showed this...
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1. Methods have been described for reducing protein S-S groups, for oxidizing protein SH groups, and for estimating protein S-S and SH groups. 2. It has been found necessary in estimating the cystine content of proteins by the Folin-Marenzi method to take into account any cysteine that may be present. 3. A method for estimating the cysteine content of proteins has been described. 4. With these ...
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Whole cells and isolated outer membrane from Vibrio cholerae (Classical, Inaba) were radiolabeled with Iodogen or Iodo-beads as catalyst. Radiolabeling of whole cells was shown to be surface specific by sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis of whole cells and cell fractions. Surface-labeled whole cells regularly showed 16 distinguishable protein species, of which nine w...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1975
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90332-2